One of the new civs will be the the Spaniards which will be ruled by Queen Isabella in the game. The other new civ is the Incans with Pachacuti as their leader. The new scenario will have the player take on Christopher Columbus' journey to the New World in 1492. Both the new civs and the new scenario will be made available to download for $7.49. You can check out more info about the DLC release after the jump:
[Via email press release]
The year is 1492. To the courts of England, France, and Spain come intrepid explorers, anxious to voyage across the Atlantic in search of a western route to China and the Indies. Will they find a new trade route and uncover untold riches from the Asian shores? Or will they perhaps run aground on undiscovered lands along their way? Will native populations be receptive to the knowledge and trade prospects presented to them, or will they fight against the invaders of their homeland? What riches, fame, and opportunities await those who brave the unknown waters? What path will you choose? Who will prevail in the struggle for supremacy over this New World?
Spain – Isabella
Isabella I was the Queen of Castile and Leon for 30 years, and with her husband Ferdinand, laid the groundwork for the consolidation of Spain. For her role in the Spanish unification, patronage of Columbus' voyages to America, and ending of the Reconquista (Recapturing) of the Iberian Peninsula, Isabella is regarded as one of the most beloved and important monarchs in the Spanish crown.
Inca – Pachacuti
Pachacuti was the ninth ruler of the Kingdom of Cusco, who during his reign expanded the tiny kingdom into an expansive empire - Tawantinsuyu. Pachacuti's Incan Empire stretched from modern-day Chile to Ecuador, including most of Peru, Bolivia, and northern Argentina, and laid the foundation for an even larger Incan Empire to come.